I ran a little test to make sure the ESP-12E is able to communicate with the ATtiny85 via I2C. These are the connections required: D1 and D2 are both pulled up to 3V3 on the ESP-12E with 10K resistors. The ESP-12E and the ATtiny85 are connected via different USB cables (ATtiny85 via the Arduino Uno) to the same USB hub. The sketch for the ESP-12E (I2C master): 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 #include <stdint.h> #include <Wire.h> byte tx = 0 ; void debug ( const char * format, ...) { char buf[ 256 ]; va_list ap; va_start(ap, format); vsnprintf(buf, sizeof (buf), format, ap); va_end(ap); Serial.println(buf); } void setup () { Serial.begin( 115200 ); Wire.begin(); Wire.setClockStretchLimit( 1500 ); } void loop () { Wire.beginTransmission( 0x26 ); Wire.write( ++ tx); Wire.endTransmission(); debug( "tx = %d" , tx); Wire.requestFrom